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PCR Test

Polymerase Chain Reaction (PCR Test) is a laboratory technique used to amplify a specific DNA sequence from a sample. It is a crucial tool in molecular biology and is widely used in various fields, including medical diagnostics, forensics, and research.

In PCR, a small amount of DNA is copied (amplified) many times, producing millions or billions of copies of the target DNA sequence. The process starts with heating the DNA sample to separate the two strands of the double-stranded DNA molecule. The DNA is then cooled to allow the addition of short single-stranded DNA primers, which are complementary to the ends of the target sequence, and a heat-stable DNA polymerase enzyme, which synthesizes new DNA strands using the original DNA as a template.

The DNA sample is then subjected to a series of temperature cycles consisting of heating and cooling steps, which allow the DNA to be amplified. During each cycle, the DNA is heated to a high temperature to separate the two strands, followed by cooling to a lower temperature to allow the primers to bind to the template and the polymerase to extend the primers and synthesize new DNA strands.

PCR testing is used to detect the presence of a specific DNA sequence in a sample, such as a virus or bacteria, in order to diagnose an infection. For example, the SARS-CoV-2 virus that causes COVID-19 can be detected by PCR testing of a nasopharyngeal or oropharyngeal swab. The sample is collected from the patient, and the RNA is extracted and converted to cDNA, which is then subjected to PCR amplification using specific primers that target the virus’s RNA. If the virus is present in the sample, the PCR will generate a product that can be visualized using agarose gel electrophoresis or other methods.

In conclusion, PCR is a powerful and versatile technique that has revolutionized molecular biology and has numerous applications in various fields, including medical diagnostics and research.

Frequently Asked Questions about PCR Tests

PCR Tests at TGH Urgent Care powered by Fast Track

TGH Urgent Care powered by Fast Track is an urgent care center with over 15 locations in the Tampa Bay, Florida, area and offers a range of medical services, including PCR testing, which is used to diagnose a variety of conditions, including infectious diseases like COVID-19. The tests are performed on a sample collected from the patient, such as a nasal or throat swab, and the results are usually available within a few hours to a few days.

Having multiple locations in the Tampa Bay area makes TGH Urgent Care powered by Fast Track a convenient option for patients who need PCR testing. The walk-in clinic format and extended hours of operation also make it easy for patients to get tested without having to make an appointment in advance.

In addition to PCR testing, TGH Urgent Care powered by Fast Track offers a range of other medical services, including treatment for minor illnesses and injuries, diagnostic testing, and preventive health services. They are staffed by experienced healthcare providers who are committed to providing high-quality medical care in a convenient and affordable manner.

At TGH Urgent Care powered by Fast Track, the comfort, and efficiency of our patients is of utmost importance. To ensure that we are available to meet the needs of all our patients, our facilities offer extended hours of operation. To make the process as smooth as possible, we welcome patients on a walk-in basis and attend to them in the order of their arrival. To ensure that your visit is as productive as possible, we suggest utilizing the “On my way” system to check in prior to your arrival. This will allow us to be prepared to attend to your needs promptly and provide you with the best possible care.

Sources and References

  1. Centers for Disease Control and Prevention (CDC) website: https://www.cdc.gov/
  2. World Health Organization (WHO) website: https://www.who.int/
  3. Molecular Biology of the Cell, 5th edition, by Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter
  4. Principles of Virology, 4th edition, by S. Jane Flint, Eric F. Drake, and Frederick A. Murphy
  5. PCR Technology: Principles and Applications for DNA Amplification, edited by Henry A. Erlich

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Apollo Beach Urgent Care

6182 N U.S. Hwy 41
Apollo Beach FL 33572

(813) 925-1903

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Brandon Urgent Care

799 West Lumsden Road
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813-925-1903

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Carrollwood Urgent Care

4505 Gunn Highway
Tampa FL 33624

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Downtown at The Heights Urgent Care

303 W Palm Ave
Tampa FL 33602

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Downtown on Water Street Urgent Care

564 Channelside Drive
Tampa FL 33602

(813) 925-1903

Open 7 Days a week,
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Fish Hawk Urgent Care

5464 Lithia Pinecrest Road
Lithia Florida 33547

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Land O’ Lakes (NOW OPEN)

22945 State Road 54
Lutz FL 33549

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North Dale Mabry (NOW OPEN)

13856 N Dale Mabry Hwy
Tampa FL 33618

(813) 925-1903

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Riverview Urgent Care

11406 U.S. 301 S
Riverview FL 33578

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South Tampa Urgent Care

3301 West Gandy Boulevard
Tampa FL 33611

(813) 925-1903

Open 7 Days a week,
8:00 a.m. to 8:00 p.m.

St. Petersburg 4th Street Urgent Care

4949 4th Street North
St. Petersburg FL 33703

(813) 925-1903

Open 7 Days a week,
8:00 a.m. to 8:00 p.m.

Sunlake – Lutz (Opening Soon)


(813) 925-1903

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Tampa MLK Blvd (NOW OPEN)

2810 West Martin Luther King Junior Boulevard
Tampa FL 33607

(813) 925-1903

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Seminole Urgent Care

7601 Seminole Boulevard
Seminole FL 33772

(813) 925-1903

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Trinity at Starkey Ranch

13531 State Road 54
Odessa FL 33556

(813) 925-1903

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Sun City Center Urgent Care

16521 U.S. Highway 301
Wimauma FL 33598

813-925-1903

Open 7 Days a week,
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Tyrone Urgent Care

3251 66th St N
Saint Petersburg FL 33710

(813) 925-1903

Open 7 Days a week,
8:00 a.m. to 8:00 p.m.

Tarpon Springs Urgent Care

40545 US Hwy 19 N unit a
Tarpon Springs FL 34689

(813) 925-1903

Open 7 Days a week,
8:00 a.m. to 8:00 p.m.

Wesley Chapel Urgent Care

5504 Gateway Blvd
Wesley Chapel FL 33544

(813) 925-1903

Open 7 Days a week,
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Westchase Urgent Care

11969 Sheldon Rd
Tampa FL 33626

(813) 925-1903

Open 7 Days a week,
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The wait was minimum and the PA was very understanding. The staff at the front desk could be a bit more friendly. Other than that, good overall experience.

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By far the nicest urgent care I've had the unfortunate opportunity to experience. The cleanliness and friendliness of this clinic are unmatched. - Sara S.

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The staff was wonderful, very caring and truly cared about how I was.

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You dealt with my issue promptly and efficiently and i was on my way.

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The entire staff was so friendly & helpful. Our first visit here was quick, the office was very clean & they were great with my kids. We will be back for sure!

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